Lancaster EPrints

Antiphospholipid antibodies prevent extravillous trophoblast differentiation.

Quenby, Siobhan and Mountfield, Steve and Cartwright, Judith E. and Whitley, Guy StJ. and Chamley, Larry and Vince, Gill S. (2005) Antiphospholipid antibodies prevent extravillous trophoblast differentiation. Fertility and Sterility, 83 (3). pp. 691-698. ISSN 0015-0282

Full text not available from this repository.

Abstract

Objective We investigated the hypothesis that antiphospholipid antibodies (aPL) have a detrimental effect on human extravillous trophoblast (EVT) differentiation into giant multinucleated cells “in vitro.” Design The EVT were isolated from the placental chorion using enzymatic digestion and Percoll gradient centrifugation. After 24, 36, and 48 hours in culture, giant multinuclear cells (GMC) were identified by immunohistochemistry using antibodies to cytokeratin 7 and counted. Setting An academic research laboratory. Patient(s) Placentas were donated by women having an elective cesarean section for a normal pregnancy at term. Main outcome measure(s) This model was then used to investigate the effect of two different monoclonal aPL to β2-glycoprotein 1 (IIC5 and ID2), and control mouse IgG antibody on EVT differentiation. Result(s) Freshly isolated EVT were nonproliferative but moved together losing their intervening cell walls and differentiated into GMC. Maximal numbers of GMC were detected after 48 hours of culture. The aPL, IIC5, and ID2 significantly inhibited GMC formation, whereas the mouse IgG control had no effect. Conclusion(s) Antiphospholipid antibodies can inhibit EVT differentiation and GMC formation “in vitro” suggesting that a failure of trophoblast differentiation and subsequent uteroplacental development may be an underlying pathology in antiphospholipid syndrome-associated pregnancy loss.

Item Type: Article
Journal or Publication Title: Fertility and Sterility
Additional Information: RAE_import_type : Journal article RAE_uoa_type : Allied Health Professions and Studies
Uncontrolled Keywords: Antiphospholipid antibodies ; extravillous trophoblast
Subjects: R Medicine > R Medicine (General)
Departments: Faculty of Health and Medicine > Medicine
ID Code: 2014
Deposited By: ep_importer
Deposited On: 05 Feb 2010 11:44
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 15:37
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/2014

Actions (login required)

View Item