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Characterization of human corneal stem cells by synchrotron infrared micro-spectroscopy.

Bentley, Adam J. and Nakamura, Takahiro and Hammiche, Azzedine and Pollock, Hubert M. and Martin, Francis L. and Kinoshita, Shigeru and Fullwood, Nigel J. (2007) Characterization of human corneal stem cells by synchrotron infrared micro-spectroscopy. Molecular Vision, 13. pp. 237-242. ISSN 1090-0535

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Abstract

Purpose: The purpose of this study was to use high resolution synchrotron radiation-based Fourier Transform Infrared (FTIR) micro-spectroscopy coupled with multivariate analysis to investigate the characteristics of adult stem cell (SC) and transit amplifying (TA) cell populations of the human corneal epithelium. Methods: Spectra of individual SC and TA cells in situ from cryosections of human cornea were collected using a synchrotron micro-spectroscopy facility at Daresbury laboratory, UK. Multivariate analysis and Mann Whitney U tests were used to analyse the spectral data from the SC and TA cell populations. Results: There were marked differences between the median spectra of the two cell populations. This correlated with their level of differentiation and functional specialization. Multivariate (principal component) analysis revealed that the cell populations could be segregated into distinct clusters, with only slight overlap between the two cell types. Significant (p<0.05) spectral differences were found in the spectral regions associated with nucleic acid, protein and lipids. Conclusions: Synchrotron FTIR micro-spectroscopy together with principal component analysis is able to discriminate between SC and TA cell populations. Our results also suggest a small sub-population of corneal epithelial cells in the SC niche have TA cell-like characteristics. Many of the spectral differences between the SC and TA cell populations relate to differences in nucleic acid conformation.

Item Type: Article
Journal or Publication Title: Molecular Vision
Subjects: Q Science > QC Physics
Departments: Faculty of Science and Technology > Physics
Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 18634
Deposited By: ep_ss_importer
Deposited On: 30 Oct 2008 16:52
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 15:19
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/18634

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