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Feasibility of using prokaryote biosensors to assess acute toxicity of polycyclic aromatic hydrocarbons.

Reid, Brian J. and Semple, Kirk T. and Macleod, Christopher J. and Weitz, Hedda J. and Paton, Graeme I. (1998) Feasibility of using prokaryote biosensors to assess acute toxicity of polycyclic aromatic hydrocarbons. FEMS Microbiology Letters, 169 (2). pp. 227-233. ISSN 0378-1097

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Abstract

The aim of this study was to assess the acute toxicity of polycyclic aromatic hydrocarbons using lux-marked bacterial biosensors, Standard solutions of phenanthrene, pyrene and benzo[a]pyrene were produced using 50 mM hydroxpropyl-beta-cyclodextrin solution which contained each respective polycyclic aromatic hydrocarbon at 6.25 times the aqueous solubility limit of the compound. The polycyclic aromatic hydrocarbon solutions were incubated with each of the biosensors for 280 min and the bioluminescence monitored every 20 min. Over the incubation time period, there was no significant decrease in bioluminescence in any of the biosensors tested with the exception of Rhizobium leguminosarum biovar trifolii TAl luxAB. In this series of incubations, there was a dramatic increase in bioluminescence in the presence of phenanthrene (2.5 times) and benzo[a]pyrene (3 times) above that of the background control (biosensor without polycyclic aromatic hydrocarbon) after 20 min. Over the next 3 h, bioluminescence decreased to that of the control. An ATP assay was carried out on the biosensors to assess if uncoupling of the oxidative phosphorylation mechanisms in the respiratory chain of the cells had occurred. However, it was found that the polycyclic aromatic hydrocarbons had no effect on the organisms indicating that there was no uncoupling. Additionally, mineralisation studies using C-14-labelled polycyclic aromatic hydrocarbons showed that the biosensors could not mineralise the compounds. This study has shown that the three polycyclic aromatic hydrocarbons tested are not acutely toxic to the prokaryotic biosensors tested, although acute toxicity has been shown in other bioassays. These results question the rationale for using prokaryote biosensors to assess the toxicity of hydrophobic chemicals, such as polycyclic aromatic hydrocarbons. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.}

Item Type: Article
Journal or Publication Title: FEMS Microbiology Letters
Uncontrolled Keywords: polycyclic aromatic hydrocarbon ; acute toxicity ; lux-marked bacteria ; cyclodextrin ; risk assessment
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 18599
Deposited By: Prof Kirk T. Semple
Deposited On: 24 Oct 2008 15:56
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 15:19
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/18599

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