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Assessment of acute PAH toxicity using prokaryote biosensors.

Reid, Brian J. and MacLeod, Christopher J. A. and Semple, Kirk T. and Weitz, H. J. and Paton, G. I. (1999) Assessment of acute PAH toxicity using prokaryote biosensors. In: BIOREMEDIATION TECHNOLOGIES FOR POLYCYCLIC AROMATIC HYDROCARBON COMPOUNDS, 1900-01-01.

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Abstract

The aim of this study was to assess the acute toxicity of phenanthrene, pyrene and benzo[a]pyrene using fur-marked bacterial biosensors. Standard solutions of phenanthrene, pyrene and benzo[a]pyrene were produced using 50 mM hydroxpropyl cyclodextrin (HPCD) solution. Four microorganisms containing the lux cassette were used as the test biosensors and over the incubation time period (280 min), there was no significant decrease in bioluminescence in any of the biosensors. This study has shown that the three PAHs tested are not acutely toxic to the prokaryotic biosensors, although acute toxicity has been shown in other bioassays. These results question the rationale for using prokaryote biosensors to assess the toxicity of hydrophobic compounds, such as PAHs, from more complex environmental matrices.}

Item Type: Conference or Workshop Item (Other)
Journal or Publication Title: BIOREMEDIATION TECHNOLOGIES FOR POLYCYCLIC AROMATIC HYDROCARBON COMPOUNDS
Additional Information: 5th International In Situ and On-Site Bioremediation Symposium, SAN DIEGO, CA, APR 19-22, 1999
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 18591
Deposited By: Prof Kirk T. Semple
Deposited On: 27 Oct 2008 14:18
Refereed?: No
Published?: Published
Last Modified: 27 Jul 2012 01:59
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/18591

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