Parry, Jackie D. and Holmes, A. K. and Unwin, M. E. and Laybourn-Parry, J. (2007) The use of ultrasonic imaging to evaluate the effect of protazoan grazing and movement on the topography of bacterial biofilms. Letters in Applied Microbiology, 45 (4). pp. 364-370. ISSN 0266-8254
Full text not available from this repository.Abstract
Aims: This study evaluated the effect of protozoan movement and grazing on the topography of a dual-bacterial biofilm using both conventional light microscopy and a new ultrasonic technique. Methods and Results: Coupons of dialysis membrane were incubated in Chalkley's medium for 3 days at 23°C in the presence of bacteria (Pseudomonas aeruginosa and Klebsiella aerogenes) alone, or in co-culture with the flagellate Bodo designis, the ciliate Tetrahymena pyriformis or the amoeba Acanthamoeba castellanii. Amoebic presence resulted in a confluent biofilm similar to the bacteria-only biofilm while the flagellate and ciliate created more diverse biofilm topographies comprising bacterial microcolonies and cavities. Conclusions: The four distinct biofilm topographies were successfully discerned with ultrasonic imaging and the method yielded information similar to that obtained with conventional light microscopy. Significance and Impact of the Study: Ultrasonic imaging provides a potential way forward in the development of a portable, nondestructive technique for profiling the topography of biofilms in situ, which might aid in the future management of biofouling.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Letters in Applied Microbiology |
| Uncontrolled Keywords: | Biofilm ; microcolonies ; protozoa ; topography ; ultrasonic |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Departments: | Faculty of Health and Medicine > Biomedical & Life Sciences |
| ID Code: | 11061 |
| Deposited By: | Dr Jackie Parry |
| Deposited On: | 30 Jul 2008 15:12 |
| Refereed?: | Yes |
| Published?: | Published |
| Last Modified: | 22 Nov 2012 13:15 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/11061 |
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