Parry, Jackie D. and Holmes, A. K. and Unwin, M. E. and Laybourn-Parry, J. (2007) The use of ultrasonic imaging to evaluate the effect of protazoan grazing and movement on the topography of bacterial biofilms. Letters in Applied Microbiology, 45 (4). pp. 364-370. ISSN 0266-8254Full text not available from this repository.
Aims: This study evaluated the effect of protozoan movement and grazing on the topography of a dual-bacterial biofilm using both conventional light microscopy and a new ultrasonic technique. Methods and Results: Coupons of dialysis membrane were incubated in Chalkley's medium for 3 days at 23°C in the presence of bacteria (Pseudomonas aeruginosa and Klebsiella aerogenes) alone, or in co-culture with the flagellate Bodo designis, the ciliate Tetrahymena pyriformis or the amoeba Acanthamoeba castellanii. Amoebic presence resulted in a confluent biofilm similar to the bacteria-only biofilm while the flagellate and ciliate created more diverse biofilm topographies comprising bacterial microcolonies and cavities. Conclusions: The four distinct biofilm topographies were successfully discerned with ultrasonic imaging and the method yielded information similar to that obtained with conventional light microscopy. Significance and Impact of the Study: Ultrasonic imaging provides a potential way forward in the development of a portable, nondestructive technique for profiling the topography of biofilms in situ, which might aid in the future management of biofouling.
|Journal or Publication Title:||Letters in Applied Microbiology|
|Uncontrolled Keywords:||Biofilm ; microcolonies ; protozoa ; topography ; ultrasonic|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Health and Medicine > Biomedical & Life Sciences|
|Deposited By:||Dr Jackie Parry|
|Deposited On:||30 Jul 2008 15:12|
|Last Modified:||22 Nov 2012 13:15|
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