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Tolerance to atmospheric ozone in transgenic tobacco over-expressing glutathione synthetase in plastids.

Wellburn, Florence A. M. and Creissen, Gary P. and Lake, Janice A. and Mullineaux, Phil M. and Wellburn, Alan R. (1998) Tolerance to atmospheric ozone in transgenic tobacco over-expressing glutathione synthetase in plastids. Physiologia Plantarum, 104 (4). pp. 623-629. ISSN 0031-9317

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Abstract

A cross between transgenic tobacco (Nicotiana tabacum L.) plants which over‐expressed either γ‐glutamylcysteine synthetase (cpGSHI) or glutathione synthetase (cpGSHII) in their chloroplasts was used to compare the consequences of over‐expression of components of the glutathione synthetic pathway on tolerance to atmospheric O3 or paraquat. A high proportion (50%) of those progeny which carried the cpGSHII transgene alone showed tolerance to atmospheric O3 but not to paraquat. Progeny of an additional two, independent, self‐pollinated primary transgenic lines, which segregated in a Mendelian fashion for the presence of the cpGSHII transgene and therefore included both transformed and non‐transformed (recessive, wild‐type) plants, were also challenged by fumigation with O3. Again, in both cases, about 50% of the plants expressing the epGSHII transgene were found to be O3‐tolerant on the basis of reduced ethylene emissions and increased or unchanged total pigment concentrations. However, this tolerance was not due to specific changes in stomatal densities.

Item Type: Article
Journal or Publication Title: Physiologia Plantarum
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 10969
Deposited By: Mr Richard Ingham
Deposited On: 28 Jul 2008 16:58
Refereed?: Yes
Published?: Published
Last Modified: 18 Sep 2013 16:01
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/10969

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