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Phosphoenolpyruvate carboxykinase plays a role in interactions of carbon and nitrogen metabolism during grape seed development.

Walker, Robert P. and Chen, Zhu-Hui and Técsi, László I. and Famiani, Franco and Lea, Peter John and Leegood, Richard C. (1999) Phosphoenolpyruvate carboxykinase plays a role in interactions of carbon and nitrogen metabolism during grape seed development. Planta, 210 (1). pp. 9-18. ISSN 0032-0935

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Abstract

Phosphoenolpyruvate carboxykinase (PEPCK) was shown to be present in a range of developing seeds by measurement of its activity and by immunoblotting. Its function was investigated during grape (Vitis vinifera L.) seed development. The maximum abundance of PEPCK coincided with the deposition of storage reserves. At this stage of development, immunohistochemistry showed that PEPCK was very abundant in a layer of cells located at the boundary of developing storage tissues and in the chalaza (close to the termination of the vascular supply to the seed) and was also present in the palisade layer of the seed coat (the inner layer of the outer integument). Earlier in development PEPCK was also present in the developing palisade layer and in the inner region of the nucellus which surrounds the developing endosperm. At later stages of development, PEPCK was located in the outer region of the endosperm. However, PEPCK was present in the phloem of the seed at all stages of development. Feeding of asparagine to developing grape seeds led to a strong induction of PEPCK. We suggest that, in developing grape seeds, both the chalaza and palisade tissue may distribute imported assimilates from the vasculature to the developing storage tissues and that PEPCK may play a role in the metabolism of nitrogenous assimilates during their delivery from the vasculature to the storage tissues.

Item Type: Article
Journal or Publication Title: Planta
Uncontrolled Keywords: Asparagine - Nitrogen metabolism - Phosphoenolpyruvate carboxykinase - Seed development - Vitis (seed development)
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 10966
Deposited By: Mr Richard Ingham
Deposited On: 28 Jul 2008 16:45
Refereed?: Yes
Published?: Published
Last Modified: 18 Sep 2013 16:01
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/10966

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