Criessen, Gary P. and Firmin, John and Fryer, Michael and Kular, Baldeep and Leyland, Nicola and Reynolds, Helen and Pastori, Gabriela and Wellburn, Florence A. M. and Baker, Neil and Wellburn, Alan R. and Mullineaux, Philip (1999) Elevated glutathione biosynthetic capacity in the chloroplasts of transgenic tobacco plants paradoxically causes increased oxidative stress. Plant Cell, 11 (7). pp. 1277-1291. ISSN 1040-4651Full text not available from this repository.
Glutathione (GSH), a major antioxidant in most aerobic organisms, is perceived to be particularly important in plant chloroplasts because it helps to protect the photosynthetic apparatus from oxidative damage. In transgenic tobacco plants overexpressing a chloroplast-targeted -glutamylcysteine synthetase (-ECS), foliar levels of GSH were raised threefold. Paradoxically, increased GSH biosynthetic capacity in the chloroplast resulted in greatly enhanced oxidative stress, which was manifested as light intensity–dependent chlorosis or necrosis. This phenotype was associated with foliar pools of both GSH and -glutamylcysteine (the immediate precursor to GSH) being in a more oxidized state. Further manipulations of both the content and redox state of the foliar thiol pools were achieved using hybrid transgenic plants with enhanced glutathione synthetase or glutathione reductase activity in addition to elevated levels of -ECS. Given the results of these experiments, we suggest that -ECS–transformed plants suffered continuous oxidative damage caused by a failure of the redox-sensing process in the chloroplast.
|Journal or Publication Title:||Plant Cell|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited By:||Mr Richard Ingham|
|Deposited On:||28 Jul 2008 10:10|
|Last Modified:||24 Jun 2016 01:03|
Actions (login required)