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Development of a genetically modified bacteriophage for use in tracing sources of pollution.

Daniell, T. J. and Davy, M. L. and Smith, R. J. (2000) Development of a genetically modified bacteriophage for use in tracing sources of pollution. Journal of Applied Microbiology, 88 (5). pp. 860-869. ISSN 1364-5072

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Abstract

Bacteriophage are frequently used as biotracers to identify the source of water pollutants. Genetic manipulation of bacteriophage M13mp18 has been used to enhance this technique by creating a library in which each recombinant bacteriophage genome contains a unique identification sequence. Techniques that identify a recombinant bacteriophage by the presence of the identification sequence, including polymerase chain reaction, restriction site polymorphism and plaque hybridization, have been developed. Recombinant bacteriophage can be used to test a large number of suspected sources simultaneously. The identification sequence also eliminates confusion with natural bacteriophage present in water samples. The performance of the modified bacteriophage and the techniques were assessed in simulated field trials on a restricted site carried out under a consent for environmental release of a genetically modified organism. The techniques were also field tested at sites in northwest England using wild-type M13 bacteriophage.

Item Type: Article
Journal or Publication Title: Journal of Applied Microbiology
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 10827
Deposited By: Mr Richard Ingham
Deposited On: 24 Jul 2008 15:51
Refereed?: Yes
Published?: Published
Last Modified: 18 Sep 2013 15:59
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/10827

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