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Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane.

Fornazier, R. F. and Ferreira, R. R. and Vitória, A. P. and Molina, S. M. G. and Lea, P. J. and Azevedo, R. A. (2002) Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane. Biologia Plantarum, 45 (1). pp. 91-97. ISSN 0006-3134

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Abstract

Sugar cane (Saccharum officinarum L. cv. Copersucar SP80-3280) seedlings were grown in nutrient solution with varying concentrations (0, 2 and 5 mM) of cadmium chloride for 96 h. Leaves were analysed for catalase (CAT), glutathione reductase (GR) and superoxide dismutase (SOD) activities. Although a clear effect of CdCl2 on plant growth was observed, the activity of SOD was not altered significantly. However, the CAT activity decreased as the concentration of CdCl2 increased. GR exhibits a significant increase in activity at 2 and 5 mM CdCl2. CAT and SOD isoenzymes were further characterised by analysis in non-denaturing PAGE. Activity staining for SOD revealed up to seven isoenzymes in untreated control and 2 mM CdCl2 treated plants, corresponding to Cu/Zn-SOD isoenzymes. At 5 mM CdCl2, only six Cu/Zn-SOD isoenzymes were observed. No Fe-SOD and Mn-SOD isoenzymes were detected. For CAT, one band of activity was observed.

Item Type: Article
Journal or Publication Title: Biologia Plantarum
Uncontrolled Keywords: catalase - glutathione reductase - heavy metals - phytochelatins - reactive oxygen species - Saccharum officinarum - superoxide dismutase
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 10733
Deposited By: Mr Richard Ingham
Deposited On: 23 Jul 2008 14:19
Refereed?: Yes
Published?: Published
Last Modified: 18 Sep 2013 15:57
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/10733

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