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Growth inhibition of the filamentous fungus Aspergillus nidulans by cadmium: an antioxidant enzyme approach.

Guelfi, A. and Azevedo, R. A. and Lea, P. J. and Molina, S. M. G. (2003) Growth inhibition of the filamentous fungus Aspergillus nidulans by cadmium: an antioxidant enzyme approach. Journal of General Applied Microbiology, 49 (2). pp. 63-73. ISSN 0022-1260

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Abstract

The heavy metal cadmium is very toxic to biological systems. Although its effect on the growth of microorganisms and plants has been investigated, the response of antioxidant enzymes of Aspergillus nidulans to cadmium is not well documented. We have studied the effect of cadmium (supplied as CdCl2) on catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR). 0.005 mm CdCl2 had a very slight stimulatory effect on the growth rate of A. nidulans, but at concentrations above 0.025 mm, growth was totally inhibited. The accumulation of Cd within the mycelium was directly correlated with the increase in the concentration of CdCl2 used in the treatments. Although a cadmium-stimulated increase in SOD activity was observed, there was no change in the relative proportions of the individual Mn­SOD isoenzymes. Higher concentrations of CdCl2 induced a small increase in total CAT activity, but there was a major increase in one isoenzymic form, that could be separated by gel electrophoresis. GR activity increased significantly following treatment with the highest concentration (0.05 mm) of CdCl2. The increases in SOD, CAT, and GR activities suggest that CdCl2 induces the formation of reactive oxygen species inside the mycelia of A. nidulans.

Item Type: Article
Journal or Publication Title: Journal of General Applied Microbiology
Uncontrolled Keywords: Aspergillus nidulans ; catalase ; glutathione reductase ; oxidative stress ; superoxide dismutase
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 10701
Deposited By: Mr Richard Ingham
Deposited On: 22 Jul 2008 16:35
Refereed?: Yes
Published?: Published
Last Modified: 18 Sep 2013 15:56
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/10701

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