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Seeing ‘cool’ and ‘hot’—infrared thermography as a tool for non-invasive, high-throughput screening of Arabidopsis guard cell signalling mutants.

Wang, Yibing and Holroyd, Geoffrey and Hetherington, Alistair and Ng, Carl K-Y. (2004) Seeing ‘cool’ and ‘hot’—infrared thermography as a tool for non-invasive, high-throughput screening of Arabidopsis guard cell signalling mutants. Journal of Experimental Botany, 55 (400). pp. 1187-1193. ISSN 1460-2431

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Abstract

The use of Arabidopsis mutants defective in abscisic acid (ABA) perception has been instrumental in the understanding of stomatal function, in particular, ABA signalling in guard cells. The considerable attention devoted to ABA signalling in guard cells is due in part to (1) the fundamental role of ABA in drought stress and (2) the use of a screening protocol based on the sensitivity of seed germination to ABA. Such a screen has facilitated the isolation of ABA signalling mutants with genetic lesions that exert pleiotropic effects at the whole plant level. As such, there is a requirement for new approaches to complement the seed germination screen. The recent advances made in the use of infrared thermography as a non-invasive, high-throughput tool are reviewed here and the versatility of this technique for screening Arabidopsis defective in stomatal regulation is highlighted.

Item Type: Article
Journal or Publication Title: Journal of Experimental Botany
Uncontrolled Keywords: Arabidopsis ; drought ; EMS mutants ; infrared thermography ; low humidity.
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 10685
Deposited By: Mr Richard Ingham
Deposited On: 22 Jul 2008 14:42
Refereed?: Yes
Published?: Published
Last Modified: 18 Sep 2013 15:56
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/10685

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