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Determination of aspartate kinase in maize tissues.

Ferreira, Renato Rodrigues and Vendemiatti, Ariane and Gratão, Priscila Lupino and Lea, Peter J. and Azevedo, Ricardo Antunes (2005) Determination of aspartate kinase in maize tissues. Scientia Agricola, 62 (2). pp. 184-189. ISSN 1678-992X

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Abstract

Lysine, threonine, methionine and isoleucine are synthesized from aspartate in a branched pathway in higher plants. Aspartate kinase plays a key role in the control of the aspartate pathway. The enzyme is very sensitive to manipulation and storage and the hydroxamate assay normally used to determine aspartate kinase activity has to be altered according to the plant species and tissue to be analyzed. We have optimized the assay for the determination of aspartate kinase in maize plants callus cell cultures. Among all the assay parameters tested, the concentration of ATP/Mg and temperature were critical for enzyme activity. In the case of temperature, 35°C was shown to be the optimum temperature for aspartate kinase activity.

Item Type: Article
Journal or Publication Title: Scientia Agricola
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 10634
Deposited By: Mr Richard Ingham
Deposited On: 21 Jul 2008 14:07
Refereed?: Yes
Published?: Published
Last Modified: 24 Oct 2013 13:25
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/10634

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