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Cuticular permeance in relation to wax and cutin development along the growing barley ( Hordeum vulgare ) leaf.

Richardson, Andrew and Wojciechowski, Tobias and Franke, Rochus and Schreiber, Lukas and Kerstiens, Gerhard and Jarvis, Mike and Fricke, Wieland (2007) Cuticular permeance in relation to wax and cutin development along the growing barley ( Hordeum vulgare ) leaf. Planta, 225 (6). pp. 1471-1481. ISSN 0032-0935

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Abstract

The developing leaf three of barley provides an excellent model system for the direct determination of relationships between amounts of waxes and cutin and cuticular permeance. Permeance of the cuticle was assessed via the time-course of uptake of either toluidine blue or 14C-labelled benzoic acid ([14C] BA) along the length of the developing leaf. Toluidine blue uptake only occurred within the region 0–25 mm from the point of leaf insertion (POLI). Resistance—the inverse of permeance—to uptake of [14C] BA was determined for four leaf regions and was lowest in the region 10–20 mm above POLI. At 20–30 and 50–60 mm above POLI, it increased by factors of 6 and a further 32, respectively. Above the point of emergence of leaf three from the sheath of leaf two, which was 76–80 mm above POLI, resistance was as high as at 50–60 mm above POLI. GC-FID/MS analyses of wax and cutin showed that: (1) the initial seven fold increase in cuticular resistance coincided with increase in cutin coverage and appearance of waxes; (2) the second, larger and final increase in cuticle resistance was accompanied by an increase in wax coverage, whereas cutin coverage remained unchanged; (3) cutin deposition in barley leaf epidermis occurred in parallel with cell elongation, whereas deposition of significant amounts of wax commenced as cells ceased to elongate.

Item Type: Article
Journal or Publication Title: Planta
Uncontrolled Keywords: cuticular permeance ; cutin ; epidermal cell development ; Hordeum ; leaf growth ; transpirational water loss ; wax ; CLIVIA-MINIATA REG ; PRUNUS LAUROCERASUS L ; PLANT CUTICLES ; WATER PERMEABILITY ; BARRIER PROPERTIES ; FINE-STRUCTURE ; LEAVES ; ARABIDOPSIS ; TRANSPIRATION ; ELONGATION
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 10558
Deposited By: Dr Gerhard Kerstiens
Deposited On: 18 Jul 2008 11:45
Refereed?: Yes
Published?: Published
Last Modified: 17 Sep 2013 10:54
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/10558

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