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Saccharopine Dehydrogenase Activity in the High-Lysine Opaque and Floury Maize Mutants.

Pompeu, Georgia Bertoni and Vendemiatti, Ariane and Gratão, Priscila Lupino and Gaziola, Salete Aparecida and Lea, Peter John and Azevedo, Ricardo Antunes (2006) Saccharopine Dehydrogenase Activity in the High-Lysine Opaque and Floury Maize Mutants. Food Biotechnology, 20 (1). pp. 55-64. ISSN 0890-5436

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Abstract

Lysine is an essential amino acid normally present in very low concentration in cereal seeds. In previous reports we have studied the metabolism of lysine in several distinct high-lysine maize mutants and observed drastic variations in the activity of saccharopine dehydrogenase (SDH), a key enzyme involved in lysine degradation. We have now analyzed the activity of SDH using non-denaturing polyacrylamide gel electrophoresis (PAGE) to identify possible isoenzymes that could explain the patterns of activity previously observed. The results indicated the presence of at least two SDH isoenzymes, one contributing to approximately 90% of the total enzyme activity and a minor form only present in the wild type lines and the opaque-1 mutant. The results suggest that the differences in total SDH activity among the genotypes tested are due to alterations in the predominant SDH isoenzymic form, which is likely to be the bifunctional polypeptide containing lysine 2-oxoglutarate reductase.

Item Type: Article
Journal or Publication Title: Food Biotechnology
Uncontrolled Keywords: Amino acids ; Lysine ; Mutants ; Saccharopine dehydrogenase
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 10553
Deposited By: Mr Richard Ingham
Deposited On: 17 Jul 2008 13:29
Refereed?: Yes
Published?: Published
Last Modified: 17 Sep 2013 10:53
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/10553

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