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The use of PFGE and fglagellin gene typing in identifying clonal groups of Campylobacter jejuni in farm and clinical environments.

Fitzgerald, Collette and Stanley, Karen and Andrew, Sarah and Jones, Keith (2001) The use of PFGE and fglagellin gene typing in identifying clonal groups of Campylobacter jejuni in farm and clinical environments. Applied and Environmental Microbiology, 67 (4). pp. 1429-1436. ISSN 0099-2240

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Abstract

Although campylobacters have been isolated from a wide range of animal hosts, the association between campylobacters isolated from humans and animals in the farm environment is unclear. We used flagellin gene typing and pulsed-field gel electrophoresis (PFGE) to investigate the genetic diversity among isolates from animals (cattle, sheep, and turkey) in farm environments and sporadic cases of campylobacteriosis in the same geographical area. Forty-eight combined fla types were seen among the 315 Campylobacter isolates studied. Six were found in isolates from all four hosts and represented 50% of the total number of isolates. Seventy-one different SmaI PFGE macrorestriction profiles (mrps) were observed, with 86% of isolates assigned to one of 29 different mrps. Fifty-seven isolates from diverse hosts, times, and sources had an identical SmaI mrp and combined fla type. Conversely, a number of genotypes were unique to a particular host. We provide molecular evidence which suggests a link between campylobacters in the farm environment with those causing disease in the community.

Item Type: Article
Journal or Publication Title: Applied and Environmental Microbiology
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 10478
Deposited By: Dr Keith Jones
Deposited On: 16 Jul 2008 08:57
Refereed?: Yes
Published?: Published
Last Modified: 17 Sep 2013 10:52
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/10478

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