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DNA polymerase B is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts

Allinson, Sarah L. and Dianova, Irina I. and Dianov, Grigory L. (2001) DNA polymerase B is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts. EMBO Journal, 20 (23). pp. 6919-6926. ISSN 0261-4189

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Abstract

The repair of oxidative base lesions in DNA is a coordinated chain of reactions that includes removal of the damaged base, incision of the phosphodiester backbone at the abasic sugar residue, incorporation of an undamaged nucleotide and sealing of the DNA strand break. Although removal of a damaged base in mammalian cells is initiated primarily by a damage-specific DNA glycosylase, several lyases and DNA polymerases may contribute to the later stages of repair. DNA polymerase (Pol ) was implicated recently as the major polymerase involved in repair of oxidative base lesions; however, the identity of the lyase participating in the repair of oxidative lesions is unclear. We studied the mechanism by which mammalian cell extracts process DNA substrates containing a single 8-oxoguanine or 5,6-dihydrouracil at a defined position. We find that, when repair synthesis proceeds through a Pol -dependent single nucleotide replacement mechanism, the 5'-deoxyribosephosphate lyase activity of Pol is essential for repair of both lesions.

Item Type: Article
Journal or Publication Title: EMBO Journal
Uncontrolled Keywords: base excision repair ; cell extracts ; DNA polymerase ; NTH1 ; OGG1
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 10164
Deposited By: Dr Sarah Allinson
Deposited On: 08 Jul 2008 11:58
Refereed?: Yes
Published?: Published
Last Modified: 17 Sep 2013 10:47
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/10164

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