Allinson, Sarah L. and Dianova, Irina I. and Dianov, Grigory L. (2001) DNA polymerase B is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts. EMBO Journal, 20 (23). pp. 6919-6926. ISSN 0261-4189Full text not available from this repository.
The repair of oxidative base lesions in DNA is a coordinated chain of reactions that includes removal of the damaged base, incision of the phosphodiester backbone at the abasic sugar residue, incorporation of an undamaged nucleotide and sealing of the DNA strand break. Although removal of a damaged base in mammalian cells is initiated primarily by a damage-specific DNA glycosylase, several lyases and DNA polymerases may contribute to the later stages of repair. DNA polymerase (Pol ) was implicated recently as the major polymerase involved in repair of oxidative base lesions; however, the identity of the lyase participating in the repair of oxidative lesions is unclear. We studied the mechanism by which mammalian cell extracts process DNA substrates containing a single 8-oxoguanine or 5,6-dihydrouracil at a defined position. We find that, when repair synthesis proceeds through a Pol -dependent single nucleotide replacement mechanism, the 5'-deoxyribosephosphate lyase activity of Pol is essential for repair of both lesions.
|Journal or Publication Title:||EMBO Journal|
|Uncontrolled Keywords:||base excision repair ; cell extracts ; DNA polymerase ; NTH1 ; OGG1|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Health and Medicine > Biomedical & Life Sciences|
|Deposited By:||Dr Sarah Allinson|
|Deposited On:||08 Jul 2008 11:58|
|Last Modified:||13 Feb 2016 01:02|
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