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Poly(ADP-ribose) polymerase in base excision repair: always engaged, but not essential for DNA damage processing.

Allinson, Sarah L. and Dianova, Irina I. and Dianov, Grigory L. (2003) Poly(ADP-ribose) polymerase in base excision repair: always engaged, but not essential for DNA damage processing. Acta Biochimica Polonica, 50 (1). pp. 169-179. ISSN 0001-527X

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Abstract

Poly(ADP-ribose) polymerase (PARP-1) is an abundant nuclear protein with a high affinity for single- and double-strand DNA breaks. Its binding to strand breaks promotes catalysis of the covalent modification of nuclear proteins with poly(ADP-ribose) synthesised from NAD+. PARP-1-knockout cells are extremely sensitive to alkylating agents, suggesting the involvement of PARP-1 in base excision repair; however, its role remains unclear. We investigated the dependence of base excision repair pathways on PARP-1 and NAD+ using whole cell extracts derived from normal and PARP-1 deficient mouse cells and DNA substrates containing abasic sites. In normal extracts the rate of repair was highly dependent on NAD+. We found that in the absence of NAD+ repair was slowed down 4–6-fold after incision of the abasic site. We also established that in extracts from PARP-1 deficient mouse cells, repair of both regular and reduced abasic sites was increased with respect to normal extracts and was NAD+-independent, suggesting that in both short- and long-patch BER PARP-1 slows down, rather than stimulates, the repair reaction. Our data support the proposal that PARP-1 does not play a major role in catalysis of DNA damage processing via either base excision repair pathway.

Item Type: Article
Journal or Publication Title: Acta Biochimica Polonica
Uncontrolled Keywords: DNA repair ; base excision repair ; PARP ; abasic sites ; in vitro repair ; cell extracts
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 10162
Deposited By: Dr Sarah Allinson
Deposited On: 08 Jul 2008 11:58
Refereed?: Yes
Published?: Published
Last Modified: 17 Sep 2013 10:47
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/10162

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